Journal: British Journal of Pharmacology

Article Title: The role of the gasotransmitter hydrogen sulfide in pathological calcification

doi: 10.1111/bph.14772

Figure Lengend Snippet: Cell types able to calcify and associated mechanisms of calcification

Article Snippet: We will focus below on the pathological conditions in which ectopic calcifications occur in vasculature, cartilage, and kidney, and then we will review what is known about the role of H 2 S on these ectopic calcifications. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Cell type Model/disease Species Mechanism of calcification Reference ECs PCa metastasis samples and PCa cell line overexpressing BMP4 Human ECs transition into osteoblasts Lin et al. ( 2017 ) Mouse ECs recruited by PCa xenograft Mouse Tissues from patients with FOP Human ECs transition into osteoblasts and chondrocytes Medici et al. ( 2010 ) Transgenic mice expressing constitutively active ALK2 Mouse Matrix Gla −/− Mouse ECs transition into osteoblasts due to BMP signalling Yao et al. ( 2013 ) Model of diabetes In vitro high‐glucose conditions Human Aortic ECs transition to chondrocytes Tang et al. ( 2012 ) Epithelial cells Healthy and cataractous lenses Human Lens epithelial cells transition into osteoblasts Balogh et al. ( 2016 ) In vitro pro‐calcifying medium Mouse Calcifying odontogenic tumour Human Epithelial cells showing intramatrix calcifications Bouckaert, Raubenheimer, and Jacobs ( 2000 ) Pericytes In vivo femur periosteal formation Rat Pericytes transition to osteoblasts Brighton et al. ( 1992 ) In vitro pro‐calcifying medium Bovine Pericytes express chondrocyte markers Sox9, aggrecan, and type II collagen Farrington‐Rock et al. ( 2004 ) In vivo pericytes implanted into athymic mice Mouse Neurons Electrical stimulation in brain Cat HA deposition in neuron cytoplasm Agnew, Yuen, Bullara, Jacques, and Pudenz ( 2016 ) Primary familial brain calcification SLC20A2 and PDGFRB mutations Human HA deposition in vascular tissue and brain parenchyma Lemos, Ferreira, Keasey, and Oliveira ( 2013 ) Clinical cases of different diseases Human Non‐explained mineralization of brain arteries and parenchyma McCartney and Squier ( 2014 ) Fibroblast In vitro standard medium Human Vesicles required for mineralization Yajima, Kumegawa, and Hiramatsu ( 1984 ) In vitro pro‐calcifying medium Human Skin‐derived fibroblast precursor cells transition into osteoblast Buranasinsup, Sila‐Asna, Bunyaratvej, and Bunyaratvej ( 2006 ) Pseudoxanthoma elasticum Human Fibroblast transition into the osteogenic lineage Okubo et al. ( 2017 ) VSMCs Review calcification mechanism — VSMC differentiation to osteoblastic‐like cells and matrix vesicle formation Leopold ( 2015 ) Adipocytes In vitro pro‐calcifying medium Mouse Adipocyte transition to osteoblast like phenotype Chen, O'Neill, Akl, and Moe ( 2014 ) Synoviocytes In vitro pro‐calcifying medium Human OA and RA fibroblast‐like synoviocytes express markers such as Runx2.

Techniques: Transgenic Assay, Expressing, In Vitro, In Vivo